Motorists are now paying an extra 2.12p per litre of petrol as a government fuel tax increase comes into force.
The AA, which opposed the rise, said for a family with two cars, each using an average of 1,286 litres per year, the annual tax increase will be £54.53.
The Freight Transport Association (FTA) said the rise would "push businesses over the edge".
Last year, fuel prices were at record levels, encouraging the government to delay the plans to increase duty.
The FTA said there could be further fuel tax increases this year announced in the Budget on 22 April and a rise in truck firms going out of business.
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